mouse a il1r  (Santa Cruz Biotechnology)

Journal: Cell host & microbe

Article Title: Staphylococcus aureus virulent PSMα peptides induce keratinocyte alarmin release to orchestrate IL-17-dependent skin inflammation

doi: 10.1016/j.chom.2017.10.008

Figure Lengend Snippet: A, WT, Il1r−/−, WT mice treated with IL-36R blocking antibody (IL-36RAb), and Il1r−/− mice treated with IL-36RAb were epicutaneously colonized with S. aureus for 7 days. Representative macroscopic images and HE-stained skin sections of mice colonized with S. aureus or treated with PBS (n=4 to 8 mice per group). Scale bars, 100 μm. B–E, Day 7 skin disease scores (B), S. aureus CFU in the skin (C), the number of neutrophils in the skin (D) and epidermal thickness (E) of WT, Il1r−/−, WT mice treated with IL-36RAb, and Il1r−/− mice treated with IL-36RAb (n=4 to 8 mice per group). WT mice treated with PBS are shown for comparison. Each dot represents a mouse (B, C). Data are presented as mean ± SD (D, E). Data represent combined results from 3 independent experiments. ND; not detected, n.s.; not significant, *P<0.05 and **P<0.01, by Kruskal-Wallis test (B, C) or by one-way ANOVA test with Bonferroni’s correction (D, E).

Article Snippet: Mouse: Il1r −/− : B6.129S7- Il1r1 tm1Imx /J , The Jackson Laboratory , JAX: 003245.

Techniques: Blocking Assay, Staining, Comparison

Journal: Cell host & microbe

Article Title: Staphylococcus aureus virulent PSMα peptides induce keratinocyte alarmin release to orchestrate IL-17-dependent skin inflammation

doi: 10.1016/j.chom.2017.10.008

Figure Lengend Snippet: A, Production of IL-17A, IL-17F, IL-22, IFN-γ and GM-CSF by skin cells isolated from WT mice colonized with S. aureus or treated with PBS. Intracellular cytokine production was assessed in gated CD45+CD90+ cells on day 7 after pathogen colonization by flow cytometry. Representative flow cytometry profiles (left panels) and the number of cytokine-producing cells (right panel). Results in right panel represent mean ± SD of 2 experiments. B, Production of IL-17A by CD45+ cells in the skin of WT and Myd88−/− mice 7 days after epicutaneous infection. Representative flow cytometry profiles (left panels) and the number of IL-17A-producing cells (right panel). Results in right panel represent mean ± SD of 2 experiments. C, IL-17A and IL-17F production in skin tissues of WT mice, Myd88Δker mice, Il1r−/− mice, WT mice treated with IL-36RAb and Il1r−/− mice treated with IL-36RAb 7 days after pathogen colonization. WT mice treated with PBS are shown for comparison. Each dot represents a mouse. Data represent combined data of 3 independent experiments. D WT and Il17a−/−f−/− mice were epicutaneously colonized with S. aureus or treated with PBS. Representative macroscopic images and HE-stained skin sections 7 days after colonization (n=7 mice per group). Scale bars, 100 μm. E–H, Skin disease scores (E), S. aureus CFU in the skin (F), the numbers of neutrophils (G) and epidermal thickness (H) of WT and Il17a−/−f−/− mice colonized with S. aureus. WT mice treated with PBS are shown for comparison. Each dot represents a mouse (C, E, F). Data are presented as mean ± SD (A, B, G, H). Results shown represent combined data of 3 independent experiments. ND; not detected, n.s.; not significant, *P<0.05; **P<0.01, by unpaired two-tailed Mann-Whitney U test (A, B, E, F, G, H) or Kruskal-Wallis test (C).

Article Snippet: Mouse: Il1r −/− : B6.129S7- Il1r1 tm1Imx /J , The Jackson Laboratory , JAX: 003245.

Techniques: Isolation, Flow Cytometry, Infection, Comparison, Staining, Two Tailed Test, MANN-WHITNEY